Issue 4, 2012
From the journal:

Chemical Communications

Label-free fluorescence detection of the depurination activity of ribosome inactivating protein toxins†

Arun A. Tanpure,a   Pooja Pathejab  and  Seergazhi G. Srivatsan*a  

* Corresponding authors

a Indian Institute of Science Education and Research, Sai Trinity Building, Pashan, India
E-mail: srivatsan@iiserpune.ac.in
Fax: +91-20-25899790
Tel: +91-20-25908086

b Department of Biotechnology, University of Pune, India

Abstract

Development of a simple label-free fluorescence hybridization assay to monitor the depurination activity of toxic ribosome inactivating proteins by using a fluorescent ligand that specifically pseudo base pairs with a cytosine residue opposite an abasic site is described. This method could be potentially implemented in screening platforms for the discovery of small molecules that inhibit the activity of these toxins.

Graphical abstract: Label-free fluorescence detection of the depurination activity of ribosome inactivating protein toxins

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C1CC16667K
Article type
Communication
Submitted
27 oct. 2011
Accepted
10 nov. 2011
First published
15 nov. 2011

Chem. Commun., 2012,48, 501-503

Permissions

Request permissions

Label-free fluorescence detection of the depurination activity of ribosome inactivating protein toxins

A. A. Tanpure, P. Patheja and S. G. Srivatsan, Chem. Commun., 2012, 48, 501 DOI: 10.1039/C1CC16667K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements