This Perspective reviews enzyme-activity imaging probes, centering on proteases and kinases, their architectures, and readout platforms. It underscores selectivity gains, theranostic designs, AI-assisted discovery, and growing clinical translation.
This review summarizes the design principles, activation mechanisms, and applications of stimuli-activatable covalent probes for protein labeling. It also presents challenges and perspectives on advancing these probes toward clinical translation.
Viral infections are one of the leading causes of acute morbidity in humans and much endeavour has been made by the synthetic community for the development of drugs to treat associated diseases.
An AND-gate bioluminescent probe FK-Luc-BH was constructed by integrating excitation-free BLI modality with a dual-locked strategy to monitor the co-localization of cathepsin L and hypochlorite in tumor sites for precise tumor imaging.
Enzyme-responsive targeted nanomedicines have emerged as a promising approach for precision cancer therapy, capitalizing on the unique biochemical features of the tumor microenvironment (TME) to achieve localized and controlled drug release.