Non-native Pathway Engineering with CRISPRi for Carbon Dioxide Assimilation and Valued 5-Aminolevulinic Acid Synthesis in Escherichia coli Nissle

Sefli Sri Wahyu Effendi and I-Son Ng

ACS Synth. Biol., 2024, 13, 2038
DOI: 10.1021/acssynbio.4c00318

Enhanced recombinant carbonic anhydrase in T7RNAP-equipped Escherichia coli W3110 for carbon capture storage and utilization (CCSU)

Wan-Wen Ting, Jie-Yao Yu, Yu-Chieh Lin and I-Son Ng

Bioresource Technology, 2022, 363, 128010
DOI: 10.1016/j.biortech.2022.128010

Symbiosis culture of probiotic Escherichia coli Nissle 1917 and Lactobacillus rhamnosus GG using lactate utilization protein YkgG

Yu-Chi Tseng, Chengfeng Xue and I-Son Ng

Process Biochemistry, 2023, 126, 163
DOI: 10.1016/j.procbio.2023.01.011

Challenges and opportunities of bioprocessing 5-aminolevulinic acid using genetic and metabolic engineering: a critical review

Ying-Chen Yi, I-Tai Shih, Tzu-Hsuan Yu, Yen-Ju Lee and I-Son Ng

Bioresour. Bioprocess., 2021, 8
DOI: 10.1186/s40643-021-00455-6

Precise measurement of decarboxylase and applied cascade enzyme for simultaneous cadaverine production with carbon dioxide recovery

Yu-Chieh Lin, Chengfeng Xue, Shih-I Tan, Wan-Wen Ting, Shih-Chen Yang and I-Son Ng

Journal of the Taiwan Institute of Chemical Engineers, 2022, 137, 104188
DOI: 10.1016/j.jtice.2021.104188

Carbon recycling with synthetic CO2 fixation pathways

Pei-Ru Chen and Peng-Fei Xia

Current Opinion in Biotechnology, 2024, 85, 103023
DOI: 10.1016/j.copbio.2023.103023

Engineering RuBisCO-based shunt for improved cadaverine production in Escherichia coli

Jia Feng, Ye Han, Shuang Xu, Yang Liao, Yongtao Wang, Sheng Xu, Hui Li, Xin Wang and Kequan Chen

Bioresource Technology, 2024, 398, 130529
DOI: 10.1016/j.biortech.2024.130529

Effective whole cell biotransformation of arginine to a four-carbon diamine putrescine using engineered Escherichia coli

Shih-Chen Yang, Wan-Wen Ting and I-Son Ng

Biochemical Engineering Journal, 2022, 185, 108502
DOI: 10.1016/j.bej.2022.108502

Polyhydroxyalkanoates bioproduction from bench to industry: Thirty years of development towards sustainability

Priskila Adjani Diankristanti, Yu-Chieh Lin, Ying-Chen Yi and I-Son Ng

Bioresource Technology, 2024, 393, 130149
DOI: 10.1016/j.biortech.2023.130149

Utilizing Ribose 1,5-Bisphosphate Isomerase and RuBisCO-Equipped Escherichia coli Nissle for Low-Carbon Footprint GABA Production

Sefli Sri Wahyu Effendi, Yoshihiro Toya, Hiroshi Shimizu, Chengfeng Xue and I-Son Ng

ACS Sustainable Chem. Eng., 2024, 12, 11093
DOI: 10.1021/acssuschemeng.4c03537

Applications of Synthetic Biotechnology on Carbon Neutrality Research: A Review on Electrically Driven Microbial and Enzyme Engineering

Xiaoyan Zhuang, Yonghui Zhang, An-Feng Xiao, Aihui Zhang and Baishan Fang

Front. Bioeng. Biotechnol., 2022, 10
DOI: 10.3389/fbioe.2022.826008

Effective 5‐aminolevulinic acid production via T7 RNA polymerase and RuBisCO equipped Escherichia coli W3110

Wan‐Wen Ting and I‐Son Ng

Biotech & Bioengineering, 2023, 120, 583
DOI: 10.1002/bit.28273

Adaptive laboratory evolution and metabolic regulation of genetic Escherichia coli W3110 toward low-carbon footprint production of 5-aminolevulinic acid

Wan-Wen Ting and I-Son Ng

Journal of the Taiwan Institute of Chemical Engineers, 2022, 141, 104612
DOI: 10.1016/j.jtice.2022.104612

Cell-free biocatalysis coupled with photo-catalysis and electro-catalysis: Efficient CO2-to-chemical conversion

Junzhu Yang, Chi-Kit Sou and Yuan Lu

Green Energy & Environment, 2024, 9, 1366
DOI: 10.1016/j.gee.2023.10.002

The potential of RuBisCO in CO2 capture and utilization

Kamyll Dawn Cocon and Patricia Luis

Progress in Energy and Combustion Science, 2024, 105, 101184
DOI: 10.1016/j.pecs.2024.101184

Towards high-level protein, beta-carotene, and lutein production from Chlorella sorokiniana using aminobutyric acid and pseudo seawater

Chiau-Sin Teng, Chengfeng Xue, Jia-Yi Lin and I.-Son Ng

Biochemical Engineering Journal, 2022, 184, 108473
DOI: 10.1016/j.bej.2022.108473

Toward Low-Carbon-Footprint Glycolic Acid Production for Bioplastics through Metabolic Engineering in Escherichia coli

Ying-Chen Yi and I-Son Ng

ACS Sustainable Chem. Eng., 2023, 11, 815
DOI: 10.1021/acssuschemeng.2c06718

Engineering a solar formic acid/pentose (SFAP) pathway in Escherichia coli for lactic acid production

Yajing Zhang, Tao Sun, Linqi Liu, Xupeng Cao, Weiwen Zhang, Wangyin Wang and Can Li

Metabolic Engineering, 2024, 83, 150
DOI: 10.1016/j.ymben.2024.04.002

Tailoring Genetic Elements of the Plasmid-Driven T7 System for Stable and Robust One-Step Cloning and Protein Expression in Broad Escherichia coli

Shih-I Tan, Chuan-Chieh Hsiang and I-Son Ng

ACS Synth. Biol., 2021, 10, 2753
DOI: 10.1021/acssynbio.1c00361

Recent Advances in Engineering Heterotrophic Microorganisms for Reinforcing CO2 Fixation Based on Calvin–Benson–Bassham Cycle

Jia Feng, Ding Ma, Siyuan Gao, Yang Liao, Jiao Feng, Sheng Xu, Xin Wang and Kequan Chen

ACS Sustainable Chem. Eng., 2023, 11, 9509
DOI: 10.1021/acssuschemeng.2c06627

From Rags to Riches: Exploiting the Calvin‐Benson‐Bassham Cycle for Biomanufacturing

Filippo Federici, Enrico Orsi and Pablo Iván Nikel

ChemCatChem, 2023, 15
DOI: 10.1002/cctc.202300746

Low-Carbon-Footprint Production of High-End 5-Aminolevulinic Acid via Integrative Strain Engineering and RuBisCo-Equipped Escherichia coli

Ying-Chen Yi, Chengfeng Xue and I-Son Ng

ACS Sustainable Chem. Eng., 2021, 9, 15623
DOI: 10.1021/acssuschemeng.1c05994

Investigation of enzymatic quality and quantity using pyridoxal 5′-phosphate (PLP) regeneration system as a decoy in Escherichia coli

Chengfeng Xue and I-Son Ng

International Journal of Biological Macromolecules, 2023, 235, 123814
DOI: 10.1016/j.ijbiomac.2023.123814

Metabolic Engineering of E. coli for Enhanced Diols Production from Acetate

Luca Ricci, Xuecong Cen, Yuexuan Zu, Giacomo Antonicelli, Zhen Chen, Debora Fino, Fabrizio C. Pirri, Gregory Stephanopoulos, Benjamin M. Woolston and Angela Re

ACS Synth. Biol., 2025, 14, 1204
DOI: 10.1021/acssynbio.4c00839