Issue 38, 2022

The role of nanopores constructed on the micropitted titanium surface in the immune responses of macrophages and the potential mechanisms

Abstract

The delayed transition of macrophages (MΦs) from pro-inflammatory M1 to the pro-healing M2 state on implant surfaces is one of the most important reasons for poor osseointegration. This work reports the construction of closely packed nanopores with a small diameter on the micropitted titanium (Ti) surface by two-step acid etching to promote the M1-to-M2 transition of MΦs and pays special attention to the potential mechanisms by which the nanopores decorating the micropits exert immunomodulatory effects. The results show that the structure composed of hybrid nanopores (10–25 nm) and micropits (5–15 μm) can be produced on the Ti surface by a two-step acid etching process. Compared with the unitary micropits, the micropit/nanopore surface could facilitate the switch of MΦs from the pro-inflammatory M1 to the pro-healing M2 phenotype. RNA sequencing reveals that the MAPK, PI3K-AKT and C-type lectin signaling pathways play key roles in the micro/nano-structure-mediated transition. In addition, the micro/nano-structured surface down-regulated CYP1A2 expression, reducing the generation of mitochondrial ROS, in turn restraining the oxidative stress and further attenuating inflammation. This work provides novel insights into the underlying mechanisms of immunomodulation by the nano-structure-decorated micro-structures on the Ti surface, which is significant for designing the surface of orthopedic implants from the perspective of immunomodulation.

Graphical abstract: The role of nanopores constructed on the micropitted titanium surface in the immune responses of macrophages and the potential mechanisms

Supplementary files

Article information

Article type
Paper
Submitted
18 jun. 2022
Accepted
25 ago. 2022
First published
26 ago. 2022

J. Mater. Chem. B, 2022,10, 7732-7743

The role of nanopores constructed on the micropitted titanium surface in the immune responses of macrophages and the potential mechanisms

R. Hang, Y. Zhao, Y. Zhang, R. Yao, X. Yao, Y. Sun, D. Huang and R. Hang, J. Mater. Chem. B, 2022, 10, 7732 DOI: 10.1039/D2TB01263D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements