Simultaneous determination of 169 veterinary drugs in chicken eggs with EMR-Lipid clean-up using ultra-high performance liquid chromatography tandem mass spectrometry

Abstract

A multiresidue method for the simultaneous determination of 169 veterinary drugs in chicken egg was developed and validated according to the requirements of the China Conformity Assessment GB/T 27417-2017. The method was developed by extraction using acetonitrile with 5% formic acid and then using enhanced matrix removal-lipid in dispersive solid phase extraction and drying pouches for further clean-up with ultra-high performance liquid chromatography tandem mass spectrometry of an electrospray–ionization interface in positive ion mode. The limits of detection and quantification of the analytes were 0.01–3.81 μg kg⁻¹ and 0.03–12.57 μg kg⁻¹, respectively. For the analytes, the recoveries were 57–124%, and the relative standard deviations of all analytes ranged from 2% to 31%. Linearity was evaluated by performing matrix-matched calibration at 0.1, 0.2, 0.5, 1, 5, 10, 20, and 40 μg kg⁻¹ levels. The evaluated method enabled the rapid, reliable, and robust quantification and identification of 169 veterinary drug residues and was successfully applied in real samples.