Issue 10, 2024

Bioactive components and mechanisms of Pu-erh tea in improving levodopa metabolism in rats through COMT inhibition

Abstract

Catechol-O-methyltransferase (COMT) plays a central role in the metabolic inactivation of endogenous neurotransmitters and xenobiotic drugs and hormones having catecholic structures. Its inhibitors are used in clinical practice to treat Parkinson's disease. In this study, a fluorescence-based visualization inhibitor screening method was developed to assess the inhibition activity on COMT both in vitro and in living cells. Following the screening of 94 natural products, Pu-erh tea extract exhibited the most potent inhibitory effect on COMT with an IC50 value of 0.34 μg mL−1. In vivo experiments revealed that Pu-erh tea extract substantially hindered COMT-mediated levodopa metabolism in rats, resulting in a significant increase in levodopa levels and a notable decrease in 3-O-methyldopa in plasma. Subsequently, the chemical components of Pu-erh tea were analyzed using UHPLC-Q-Exactive Orbitrap HRMS, identifying 24 major components. Among them, epigallocatechin gallate, gallocatechin gallate, epicatechin gallate, and catechin gallate exhibited potent inhibition of COMT activity with IC50 values from 93.7 nM to 125.8 nM and were the main bioactive constituents in Pu-erh tea responsible for its COMT inhibition effect. Inhibition kinetics analyses and docking simulations revealed that these compounds competitively inhibit COMT-mediated O-methylation at the catechol site. Overall, this study not only explained how Pu-erh tea catechins inhibit COMT, suggesting Pu-erh tea as a potential dietary intervention for Parkinson's disease, but also introduced a new strategy for discovering COMT inhibitors more effectively.

Graphical abstract: Bioactive components and mechanisms of Pu-erh tea in improving levodopa metabolism in rats through COMT inhibition

Supplementary files

Article information

Article type
Paper
Submitted
31 ene. 2024
Accepted
08 abr. 2024
First published
09 abr. 2024

Food Funct., 2024,15, 5287-5299

Bioactive components and mechanisms of Pu-erh tea in improving levodopa metabolism in rats through COMT inhibition

Z. Zhou, Y. Li, F. Wang, G. Zhu, S. Qi, H. Wang, Y. Ma, R. Zhu, Y. Zheng, G. Ge and P. Wang, Food Funct., 2024, 15, 5287 DOI: 10.1039/D4FO00538D

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