Issue 48, 2023

Hierarchical peroxiredoxin assembly through orthogonal pH-response and electrostatic interactions

Abstract

Morpheeins are proteins that adapt their morphology and function to the environment. Therefore, their use in nanotechnology opens up the bottom-up preparation of anisotropic metamaterials, based on the sequential use of different stimuli. A prominent member of this family of proteins is peroxiredoxins (Prx), with dual peroxidase and chaperone function, depending on the pH of the media. At high pH, they show a toroidal morphology that turns into tubular stacks upon acidification. While the toroidal conformers have been explored as building blocks to yield 1D and 2D structures, the obtention of higher ordered materials remain unexplored. In this research, the morpheein behaviour of Prx is exploited to yield columnar aggregates, that are subsequently self-assembled into 3D anisotropic bundles. This is achieved by electrostatic recognition between the negatively charged protein rim and a positively charged porphyrin acting as molecular glue. The subsequent and orthogonal input lead to the alignment of the monodimensional stacks side-by-side, leading to the precise assembly of this anisotropic materials.

Graphical abstract: Hierarchical peroxiredoxin assembly through orthogonal pH-response and electrostatic interactions

Supplementary files

Article information

Article type
Paper
Submitted
20 feb. 2023
Accepted
13 nov. 2023
First published
14 nov. 2023
This article is Open Access
Creative Commons BY license

J. Mater. Chem. B, 2023,11, 11544-11551

Hierarchical peroxiredoxin assembly through orthogonal pH-response and electrostatic interactions

E. Anaya-Plaza, Z. Özdemir, Z. Wimmer and M. A. Kostiainen, J. Mater. Chem. B, 2023, 11, 11544 DOI: 10.1039/D3TB00369H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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