Issue 10, 2023

Biosynthesis of the fungal nonribosomal peptide penilumamide A and biochemical characterization of a pterin-specific adenylation domain

Abstract

We report the characterization of the penilumamide biosynthetic cluster from Aspergillus flavipes CNL-338. In vitro reconstitution experiments demonstrated that three nonribosomal peptide synthetases are required for constructing the tripeptide and studies with dissected adenylation domains allowed for the first biochemical characterization of a domain that selects a pterin-derived building block.

Graphical abstract: Biosynthesis of the fungal nonribosomal peptide penilumamide A and biochemical characterization of a pterin-specific adenylation domain

Supplementary files

Article information

Article type
Communication
Submitted
09 jun. 2023
Accepted
31 ago. 2023
First published
06 sep. 2023
This article is Open Access
Creative Commons BY-NC license

RSC Chem. Biol., 2023,4, 748-753

Biosynthesis of the fungal nonribosomal peptide penilumamide A and biochemical characterization of a pterin-specific adenylation domain

S. C. Heard, K. L. Diehl and J. M. Winter, RSC Chem. Biol., 2023, 4, 748 DOI: 10.1039/D3CB00088E

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