Issue 6, 2021

Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library

Abstract

CDy1 is a powerful tool to distingusih embryonic stem cells for reprogramming studies and regeneration medicine. However, the stem cell selectivity mechanism of CDy1 has not been fully understood. Here, we report ALDH2 and ABCB1 as the molecular targets of CDy1, elucidated by live-cell affinity-matrix and ABC transporter CRISPRa library screening. The two unique orthogonal mechanisms provide the potential of multi-demensional cellular distinction of specific cell types.

Graphical abstract: Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library

Supplementary files

Article information

Article type
Communication
Submitted
15 jul. 2021
Accepted
20 ago. 2021
First published
20 ago. 2021
This article is Open Access
Creative Commons BY license

RSC Chem. Biol., 2021,2, 1590-1593

Target identification of mouse stem cell probe CDy1 as ALDH2 and Abcb1b through live-cell affinity-matrix and ABC CRISPRa library

N. Miyamoto, Y. Go, L. M. Ciaramicoli, H. Kwon, H. S. Kim, X. Bi, Y. H. Yu, B. Kim, H. Ha, N. Kang, S. Yun, J. Kim, H. Cha and Y. Chang, RSC Chem. Biol., 2021, 2, 1590 DOI: 10.1039/D1CB00147G

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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