Issue 5, 2021

A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems

Abstract

Alkyne functional groups have Raman signatures in a region (1800 cm−1 to 2800 cm−1) that is free from interference from cell components, known as the “silent region”, and alkyne signals in this region were first utilized a decade ago to visualize the nuclear localization of a thymidine analogue EdU. Since then, the strategy of Raman imaging of biological samples by using alkyne functional groups, called alkyne-tag Raman imaging (ATRI), has become widely used. This article reviews the applications of ATRI in biological samples ranging from organelles to whole animal models, and briefly discusses the prospects for this technique.

Graphical abstract: A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems

Article information

Article type
Review Article
Submitted
20 may. 2021
Accepted
07 jul. 2021
First published
14 jul. 2021
This article is Open Access
Creative Commons BY license

RSC Chem. Biol., 2021,2, 1415-1429

A decade of alkyne-tag Raman imaging (ATRI): applications in biological systems

S. Bakthavatsalam, K. Dodo and M. Sodeoka, RSC Chem. Biol., 2021, 2, 1415 DOI: 10.1039/D1CB00116G

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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