Issue 9, 2017

Understanding single enzyme activity via the nano-impact technique

Abstract

To evaluate the possible detection of single enzyme activity via electrochemical methods, a combined finite difference and random walk simulation is used to model individual enzyme-electrode collisions where such events are monitored amperometrically via the measurement of products formed by the enzyme in solution. It is found that the observed signal is highly sensitive to both the enzyme turnover number, the size of the electrode and the bandwidth of the electronics. Taking single catalase impacts as an example, simulation results are compared with experimental data. Our work shows the requirement for the detection of electrochemically active product formed by individual enzymes and gives guidance for the design of experiments.

Graphical abstract: Understanding single enzyme activity via the nano-impact technique

Supplementary files

Article information

Article type
Edge Article
Submitted
09 may. 2017
Accepted
17 jul. 2017
First published
19 jul. 2017
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY license

Chem. Sci., 2017,8, 6423-6432

Understanding single enzyme activity via the nano-impact technique

C. Lin, E. Kätelhön, L. Sepunaru and R. G. Compton, Chem. Sci., 2017, 8, 6423 DOI: 10.1039/C7SC02084H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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