Image analysis for a microfluidic paper-based analytical device using the CIE L*a*b* color system

Takeshi Komatsu; Saeed Mohammadi; Lori Shayne Alamo Busa; Masatoshi Maeki; Akihiko Ishida; Hirofumi Tani; Manabu Tokeshi

doi:10.1039/C6AN01409G

Image analysis for a microfluidic paper-based analytical device using the CIE Lab* color system†

Takeshi Komatsu,^a Saeed Mohammadi,^a Lori Shayne Alamo Busa,‡^a Masatoshi Maeki,^b Akihiko Ishida,*^b Hirofumi Tani^b and Manabu Tokeshi*^bcde

Author affiliations

* Corresponding authors

^a Graduate School of Chemical Sciences and Engineering, Hokkaido University, Kita 13 Nishi8, Kita-ku, Sapporo 060-8628, Japan

^b Division of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan
E-mail: ishida-a@eng.hokudai.ac.jp
Fax: +81-11-706-6745
Tel: +81-11-706-6746

^c ImPACT Research Centre for Advanced Nanobiodevices, Nagoya University, Furo-cho Chikusa-ku, Nagoya 464-8603, Japan

^d Innovative Research Centre for Preventive Medical Engineering, Nagoya University, Furo-cho Chikusa-ku, Nagoya 464-8603, Japan

^e Institute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
E-mail: tokeshi@eng.hokudai.ac.jp
Fax: +81-11-706-6745
Tel: +81-11-706-6744

Abstract

The combination of a microfluidic paper-based analytical device (μPAD) and digital image analysis is widely used for quantitative analysis with μPADs because of its easy and simple operation. Herein, we have demonstrated a quantitative analysis based on multiple color changes on a μPAD. The CIE L*a*b* color system was employed to analyse the digital images obtained with the μPAD. We made pH measurements using a universal pH-indicator showing multiple color changes for various pH values of aqueous test solutions. The detectable pH range of this method was wider than the typical grayscale-based image analysis, and we succeeded in the measurements for a wide pH range of 2–9.

This article is part of the themed collection: Analyst Recent Open Access Articles

Supplementary files

Article information

DOI: https://doi.org/10.1039/C6AN01409G
Article type: Communication
Submitted: 21 jun. 2016
Accepted: 27 oct. 2016
First published: 28 oct. 2016
This article is Open Access

Download Citation

Analyst, 2016,141, 6507-6509

Permissions

Request permissions

Image analysis for a microfluidic paper-based analytical device using the CIE Lab* color system

T. Komatsu, S. Mohammadi, L. S. A. Busa, M. Maeki, A. Ishida, H. Tani and M. Tokeshi, Analyst, 2016, 141, 6507 DOI: 10.1039/C6AN01409G

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Analyst