A fast responsive, highly selective and light-up fluorescent probe for the two-photon imaging of carboxylesterase in living cells

Jianguo Wang; Qingqing Chen; Na Tian; Wenping Zhu; Hang Zou; Xuesong Wang; Xiaokang Li; Xiaolin Fan; Guoyu Jiang; Ben Zhong Tang

doi:10.1039/C8TB00147B

A fast responsive, highly selective and light-up fluorescent probe for the two-photon imaging of carboxylesterase in living cells†

Jianguo Wang,

^a Qingqing Chen,^a Na Tian,^b Wenping Zhu,^a Hang Zou,^c Xuesong Wang,

^b Xiaokang Li,

^a Xiaolin Fan,^a Guoyu Jiang

*^a and Ben Zhong Tang*^c

Author affiliations

* Corresponding authors

^a Key Laboratory of Organo-Pharmaceutical Chemistry, Gannan Normal University, Ganzhou 341000, P. R. China
E-mail: jiangguoyu@mail.ipc.ac.cn

^b Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China

^c Department of Chemistry, Institute for Advanced Study, Institute of Molecular Functional Materials, and State Key Laboratory of Molecular Neuroscience, The Hong Kong University of Science & Technology, Clear Water Bay, Kowloon, Hong Kong, China
E-mail: tangbenz@ust.hk

Abstract

A fast responsive and two photon fluorescent probe (HCyNAc) for carboxylesterase (CaE) has been designed based on the D–π–A structure of hemicyanine and naphthalene derivatives. After enzymatic reaction, HCyNAc enabled light-up fluorescence assay of CaE over other biologically-relevant species and enzymes, including ROS. Two-photon imaging of endogenous CaE was confirmed in HeLa cells using HCyNAc under 800 nm NIR excitation.

Journal of Materials Chemistry B

A fast responsive, highly selective and light-up fluorescent probe for the two-photon imaging of carboxylesterase in living cells†

Abstract

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A fast responsive, highly selective and light-up fluorescent probe for the two-photon imaging of carboxylesterase in living cells

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