Issue 9, 2015

A short review of applications of liquid chromatography mass spectrometry based metabolomics techniques to the analysis of human urine

Abstract

The applications of metabolomics as a methodology for providing better treatment and understanding human disease continue to expand rapidly. In this review, covering the last two years, the focus is on liquid chromatography-mass spectrometry (LC-MS) profiling of metabolites in urine. In LC-MS based metabolomics there are still problems with regard to: chromatographic separation, peak picking and alignment, metabolite identification, metabolite coverage, instrument sensitivity and data interpretation and in the case of urine sample normalisation. Progress has been made with regard to all of these issues during the period of the review. Of particular interest are the increasing use of orthogonal chromatographic methods for optimal metabolite coverage and the increasing adoption of receiver operator characteristic (ROC) curves for biomarker validation.

Graphical abstract: A short review of applications of liquid chromatography mass spectrometry based metabolomics techniques to the analysis of human urine

Article information

Article type
Minireview
Submitted
14 dic. 2014
Accepted
02 mar. 2015
First published
03 mar. 2015

Analyst, 2015,140, 2907-2915

Author version available

A short review of applications of liquid chromatography mass spectrometry based metabolomics techniques to the analysis of human urine

T. Zhang and D. G. Watson, Analyst, 2015, 140, 2907 DOI: 10.1039/C4AN02294G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements