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Themed collection Droplet-based single-cell sequencing

10 items
Editorial

Single cell sequencing: An engineer and business person's perspective on how we got here

Microfluidics entrepreneur Mark Gilligan provides a perspective on the development of single-cell sequencing technologies.

Graphical abstract: Single cell sequencing: An engineer and business person's perspective on how we got here
From the themed collection: Droplet-based single-cell sequencing
Editorial

InDrops and Drop-seq technologies for single-cell sequencing

Droplet-based single-cell sequencing pioneers Allon Klein and Evan Macosko summarise the growth of the technology and look ahead to future developments.

Graphical abstract: InDrops and Drop-seq technologies for single-cell sequencing
From the themed collection: Droplet-based single-cell sequencing
Editorial

Perspective on droplet-based single-cell sequencing

Thought leader David Weitz introduces the Lab on a Chip droplet-based single-cell sequencing thematic collection.

Graphical abstract: Perspective on droplet-based single-cell sequencing
From the themed collection: Droplet-based single-cell sequencing
Perspective

Droplet-based single cell RNAseq tools: a practical guide

A step-by-step guide for droplet-based single cell RNAseq experiments, practical considerations and technical notes.

Graphical abstract: Droplet-based single cell RNAseq tools: a practical guide
From the themed collection: Lab on a Chip Recent Review Articles
Critical Review

Finding a helix in a haystack: nucleic acid cytometry with droplet microfluidics

Nucleic acid cytometry using droplet microfluidics identifies and sorts single cells, virus, or free molecules based on specific “keyword” sequences.

Graphical abstract: Finding a helix in a haystack: nucleic acid cytometry with droplet microfluidics
From the themed collection: Lab on a Chip Recent Review Articles
Paper

High throughput gene expression profiling of yeast colonies with microgel-culture Drop-seq

We describe isogenic colony sequencing (ICO-seq), a massively-parallel strategy to assess the gene expression profiles of large numbers of genetically distinct yeast colonies.

Graphical abstract: High throughput gene expression profiling of yeast colonies with microgel-culture Drop-seq
From the themed collection: Droplet-based single-cell sequencing
Open Access Paper

Simplified Drop-seq workflow with minimized bead loss using a bead capture and processing microfluidic chip

Single-cell RNA-sequencing (scRNA-seq) has revolutionized biomedical research by enabling the in-depth analysis of cell-to-cell heterogeneity of tissues with unprecedented resolution.

Graphical abstract: Simplified Drop-seq workflow with minimized bead loss using a bead capture and processing microfluidic chip
From the themed collection: Droplet-based single-cell sequencing
Paper

Functional TCR T cell screening using single-cell droplet microfluidics

Droplet-based single cell platform allows functional screening and sorting of desirable TCR T cells to accelerate development of adoptive T cell therapies.

Graphical abstract: Functional TCR T cell screening using single-cell droplet microfluidics
From the themed collection: Immunotherapy
Paper

Ultrahigh-throughput droplet microfluidic device for single-cell miRNA detection with isothermal amplification

An ultrahigh-throughput single-cell miRNA assay is developed by a continuous-flow microfluidic process employing isothermal amplification to amplify the target miRNA signal.

Graphical abstract: Ultrahigh-throughput droplet microfluidic device for single-cell miRNA detection with isothermal amplification
From the themed collection: Droplet-based single-cell sequencing
Paper

Inertial-ordering-assisted droplet microfluidics for high-throughput single-cell RNA-sequencing

We developed a modified high-throughput droplet barcoding technique for single-cell Drop-Seq via introduction of hydrodynamic ordering in a spiral microchannel.

Graphical abstract: Inertial-ordering-assisted droplet microfluidics for high-throughput single-cell RNA-sequencing
From the themed collection: Droplet-based single-cell sequencing
10 items

About this collection

The field of droplet-based single-cell sequencing field has made increasing advances in recent years. Large numbers of studies are underway to collect and explore the new information that is now accessible with single-cell RNA-seq. Improvements to microfluidics are advancing rapidly and extensions to other sequencing methods are also being developed, enabling investigations to probe information beyond mRNA alone. This has rapidly become a burgeoning field, where microfluidic techniques are essential and where droplet-based microfluidics has enabled a major advance. The collection highlights research focussing on the interface between the technological advancements and high impact applications of droplet-based single-cell sequencing. This on-going collection is collated by Thought Leader David Weitz and the Lab on a Chip Editorial Office.

More details about the collection and how to apply can be found at rsc.li/drop-sc-seq-blog

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