Communication
P450 cyptide synthase MpoB catalyzes the cross-linking of the YPW motif on the precursor peptide
The uncharacterized cytochrome P450 cyptide synthase MpoB from Micromonospora polyrhachis DSM 45886 catalyzes a cross-link between Tyr-C3 and Trp-N1 at the YPW motif on the precursor peptide MpoA.
Communication
Efficient synthesis of O-glycosylated amino acids
Synthetic glycopeptides are versatile tools to investigate protein glycosylation. In this work, we outline a short, economical synthesis to O-glycosylated amino acids compatible with Fmoc-SPPS.
Paper
Sources of mismeasurement of RNA knockdown by DNAzymes and XNAzymes
Nucleic acid catalysts can carry over into cellular RNA preparations following transfection, causing false positive and false negative knockdown measurements.
Paper
The impact of conjugation strategies and linker density on the performance of the Spermine-AcDex nanoparticle–splenocyte conjugate
A common approach in living medicine engineering is modifying cell surfaces with nanomedicines. The choice of conjugation strategy and the chemical structure of the linker are crucial for living medicine design.
Paper
Discovery of an exquisitely selective WDR5 chemical probe accelerated by a high-quality DEL–ML Hit
LH168, a potent and highly selective chemical probe for WDR5, developed based on DEL–ML (DNA encoded library–machine learning) hit.
Paper
Photoactivatable and photolabile pharmacophores: lessons learned from capsaicin
Structurally merging light-susceptible with bioactive molecules allows to generate photolabile chromopharmacophores. Using capsaicinoids, chromopharmacophores could activate the TRPV1 channel, while no activation is observed for the photoproducts.
Paper
Structure-guided engineering of a polyphosphate kinase 2 class III from an Erysipelotrichaceae bacterium to produce base-modified purine nucleotides
The polyphosphate kinase 2 class III from an Erysipelotrichaceae bacterium is highly promiscuous. We determined the first crystal structure of the enzyme and applied structure-guided engineering to enable efficient access to ATP and GTP analogues.
Paper
The SpyBLI cell-free pipeline for the rapid quantification of binding kinetics from crude samples
A less than 24-hour workflow with little hands-on time delivers quantitative protein binding kinetic data straight from expression mixtures, bypassing both binder purification and concentration determination.
About this collection
RSC Chemical Biology is proud to present this collection of invited contributions from early career researchers who are making significant contributions to the field of chemical biology. Congratulations to all of the featured researchers!
New articles will be added to this collection as they are published