Themed collection 2024 RSC Chemical Biology Emerging Investigators

This article profiles the early career researchers whose work features in the RSC Chemical Biology Emerging Investigators Collection 2024.

This work translated the preceding metal-catalyzed tryptophan modification method into a metal-free process, enabling efficient labeling of peptides, proteins, and even cell lysates in hexafluoroisopropanol (HFIP).

This work describes the rapid optimisation of a new fluorescence-based assay for RecBCD enzyme activity by integrating D-optimal design of experiments, functional data analysis, and automation.

Macrocyclic peptide ligands developed via RaPID technology selectively bind and stabilize rhGBA1 in plasma at nanomolar concentrations without inhibiting endogenous GBA1, offering potential for combinatorial ERT-pharmacological chaperone for Gaucher disease.

Our study demonstrates that encapsulating tandem nitroreductase NfsB within encapsulins, along with pore mutations, enhances enzyme activity, with cryo-EM revealing its structural architecture and multiple pore states.

Demonstrating the use of azide-containing GalNAc analogues in conjunction with an engineered metabolic pathway to study glycosylation cell-specifically.

QMP-SOs are molecular probes capable of proximity labeling of proteins in a superoxide-dependent manner. This enables fluorescence imaging of superoxide and profiling proteins associated with superoxide biology by chemoproteomics.

Unnatural base pairs (UBPs) augment the chemical diversity of artificial nucleic acids and can enable the generation of new aptamers and catalytic nucleic acids by in vitro selection. Reverse transcription of UBPs as key step during RNA in vitro selection is investigated.