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Themed collection The ‘Single’ Issue

17 items
Editorial

Editorial – Single Entities

Professor Henry S. White, Guest Editor of this themed issue on Single Entities, highlights the wealth of information that there is to be gained when analytical chemistry is studied at the “single entity” level.

Graphical abstract: Editorial – Single Entities
From the themed collection: The ‘Single’ Issue
Minireview

Sampling techniques for single-cell electrophoresis

This review covers recent advances in the sampling and loading of single cells for electrophoretic analysis by interfacing mechanical, microfluidic, and optical techniques to capillary and on-chip electrophoresis instruments.

Graphical abstract: Sampling techniques for single-cell electrophoresis
From the themed collection: The ‘Single’ Issue
Communication

Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy of a single endothelial cell

Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) with the use of a slide-on germanium accessory followed by chemometric analysis allowed for providing meaningful information about the biochemical composition of a single endothelial cell.

Graphical abstract: Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy of a single endothelial cell
From the themed collection: The ‘Single’ Issue
Communication

Highly sensitive DNA hybridization detection with single nanoparticle flash-lamp darkfield microscopy

Single AuNP counting in free solution was achieved with flash-lamp darkfield microscopy and applied to highly sensitive DNA hybridization detection based on target DNA induced AuNP aggregation and counts reduction.

Graphical abstract: Highly sensitive DNA hybridization detection with single nanoparticle flash-lamp darkfield microscopy
From the themed collection: The ‘Single’ Issue
Paper

High throughput single molecule tracking for analysis of rare populations and events

High throughput single molecule tracking methods were developed to perform quantitative analyses of rare molecular populations.

Graphical abstract: High throughput single molecule tracking for analysis of rare populations and events
From the themed collection: The ‘Single’ Issue
Paper

Using a nanopore for single molecule detection and single cell transfection

A synthetic nanopore can be used to both transfect a single cell and detect secreted analytes.

Graphical abstract: Using a nanopore for single molecule detection and single cell transfection
From the themed collection: The ‘Single’ Issue
Paper

Real-time characterization of cytotoxicity using single-cell impedance monitoring

A single-cell based platform demonstrates the ability to monitor apoptosis and necrosis of cells treated with varying concentrations of three toxicants. Individual cells showed variable responses to a given toxicant, while the averaged cellular response agrees well with those obtained from conventional cytotoxicity assays.

Graphical abstract: Real-time characterization of cytotoxicity using single-cell impedance monitoring
From the themed collection: The ‘Single’ Issue
Paper

Single nanoparticle spectroscopy for real-time in vivo quantitative analysis of transport and toxicity of single nanoparticles in single embryos

Single nanoparticle plasmonic spectroscopy for real-time in vivo probing of transport and toxicity of single NPs.

Graphical abstract: Single nanoparticle spectroscopy for real-time in vivo quantitative analysis of transport and toxicity of single nanoparticles in single embryos
From the themed collection: The ‘Single’ Issue
Paper

A hyphenated optical trap capillary electrophoresis laser induced native fluorescence system for single-cell chemical analysis

A hyphenated optical trap sampling system and a capillary electrophoresis with laser-induced native fluorescence detection system is used for sampling and characterizing the indolamines from individual pinealocytes.

Graphical abstract: A hyphenated optical trap capillary electrophoresis laser induced native fluorescence system for single-cell chemical analysis
From the themed collection: The ‘Single’ Issue
Paper

A hydrophobic entrance enhances ion current rectification and induces dewetting in asymmetric nanopores

A nanoporous system with a hydrophobic layer at the pore entrance gates transport of ions and water.

Graphical abstract: A hydrophobic entrance enhances ion current rectification and induces dewetting in asymmetric nanopores
From the themed collection: The ‘Single’ Issue
Paper

Expression of membrane-associated proteins within single emulsion cell facsimiles

This paper aims to promote the combination of microdroplet technology with cell-free cytoskeletal protein expression as tool for biological investigation.

Graphical abstract: Expression of membrane-associated proteins within single emulsion cell facsimiles
From the themed collection: The ‘Single’ Issue
Paper

Noise Adjusted Principal Component reconstruction to optimize infrared microspectroscopy of individual live cells

Presented is an optimized FTIR imaging methodology to achieve fast data acquisition with high signal-to-noise ratio for individual live cells in aqueous environments.

Graphical abstract: Noise Adjusted Principal Component reconstruction to optimize infrared microspectroscopy of individual live cells
From the themed collection: The ‘Single’ Issue
Paper

Examining changes in cellular communication in neuroendocrine cells after noble metal nanoparticle exposure

We explore the effects of noble metal nanoparticle exposure, with varied size or surface functionality, in murine chromaffin cells using microelectrode amperometry.

Graphical abstract: Examining changes in cellular communication in neuroendocrine cells after noble metal nanoparticle exposure
From the themed collection: The ‘Single’ Issue
Paper

Single molecule fluorescence correlation spectroscopy of single apoptotic cells using a red-fluorescent caspase probe

Fluorescence Correlation Spectroscopy was used to assay caspase activity in single apoptotic lymphocytes using a new red-fluorescent probe. Apoptotic cells were detected as early as 30 min after induction.

Graphical abstract: Single molecule fluorescence correlation spectroscopy of single apoptotic cells using a red-fluorescent caspase probe
From the themed collection: The ‘Single’ Issue
Paper

Metabolism of peptide reporters in cell lysates and single cells

Peptide cleavage sites occurring during incubation in lysates and single cells were identified and non-native amino acids inserted to stabilize the peptide. Multiple iterative cycles yielded a peptidase-resistant, kinase-substrate peptide.

Graphical abstract: Metabolism of peptide reporters in cell lysates and single cells
From the themed collection: The ‘Single’ Issue
Paper

Transport of redox probes through single pores measured by scanning electrochemical-scanning ion conductance microscopy (SECM-SICM)

A fast, simple method for batch fabrication of high-resolution SECM-SICM electrodes and their application in measurement of transport.

Graphical abstract: Transport of redox probes through single pores measured by scanning electrochemical-scanning ion conductance microscopy (SECM-SICM)
From the themed collection: The ‘Single’ Issue
Paper

Detection of expressed gene in isolated single cells in microchambers by a novel hot cell-direct RT-PCR method

We proposed a novel hot cell-direct RT-PCR method and demonstrated single cell RT-PCR for many isolated Jurkat cells using this method on the CD-shaped device for single cell isolation.

Graphical abstract: Detection of expressed gene in isolated single cells in microchambers by a novel hot cell-direct RT-PCR method
From the themed collection: The ‘Single’ Issue
17 items

About this collection

These papers were all invited to a themed issue on Single Entities, Guest Edited by Henry S. White from the University of Utah.

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