Top Down and Bottom Up Analysis of Proteins (Focusing on Quantitative Aspects)
Expression and Purification of Bioactive Proteins/Peptides with Conventional Liquid Chromatography
Liquid Chromatography-Mass Spectrometry of Intact Proteins
On-line Protein Digestion in Combination with Chromatographic Separation and Mass Spectrometric Detection
Bioinformatic Tools for the LC-MS/MS Analysis of Proteins and Peptides
LC-MS for the Determination of the Enzymatic Activity of Proteins
Functional Analysis of Proteins, Including LC-MS and Special Freeware
About this book
This book is the first example in presenting LC-MS strategies for the analysis of peptides and proteins with detailed information and hints about the needs and problems described from experts on-the-job. The best advantage is -for sure- the practical insight of experienced analysts into their novel protein analysis techniques. Readers starting in 'Proteomics' should be able to repeat each experiment with own equipment and own protein samples, like clean-up, direct protein analysis, after (online) digest, with modifications and others. Furthermore, the reader will learn more about strategies in protein analysis, like quantitative analysis, industrial standards, functional analysis and more.
Thomas Letzel is associate professor for analytical chemistry and bioanalysis at the Wissenschaftszentrum Weihenstephan (WZW) located at the TUM (Technische Universitõt M³nchen), Germany. He obtained his PhD in Chemistry with studies about soot aerosols and environmental analysis at the Chair for Analytical Chemistry of TUM. After a postdoc time in Amsterdam, The Netherlands, at the VU Amsterdam with topics in pharmaceutical and enzymatic analysis, he came back to TUM and was employed as a scientist, leading a new analytical research group with focus on bioanalysis. In latter position he got his Habilitation. He developed and established mass spectrometric techniques coupled with modern liquid phase solution techniques in enzymology, studying noncovalent complexes and observing molecular fingerprints. His research of novel mass spectrometric techniques comprises the identification and characterization of organic molecules in applications of biological, environmental, pharmaceutical, food, plant and nutrition sources.