Chapter 4
In-cell NMR in Eukaryotic Cells – II (Direct Protein Expression)
Enrico Luchinat and Lucia Banci
Pages 45 - 61
Chapter 6
In-cell Structure Determination Using Paramagnetic NMR Spectroscopy
Xun-Cheng Su and Thomas Huber
Pages 90 - 102
Chapter 7
Bioreactor System for In-cell NMR Observation
Noritaka Nishida and Ichio Shimada
Pages 103 - 116
Chapter 8
19F In-cell NMR
Yansheng Ye and Conggang Li
Pages 117 - 130
Chapter 9
Cellular Solid-state NMR Spectroscopy
Reinier Damman, Siddarth Narasimhan, Markus Weingarth and Marc Baldus
Pages 131 - 151
Chapter 10
In-cell EPR
Anna Bieber and Malte Drescher
Pages 152 - 169
Chapter 12
Protein Stability and Weak Intracellular Interactions
Alex J. Guseman and Gary J. Pielak
Pages 188 - 206
Chapter 13
Protein Folding, Maturation and Redox State
Enrico Luchinat and Lucia Banci
Pages 207 - 227
Chapter 14
All-atom Molecular Dynamics Simulation of Proteins in Crowded Environments
Yuji Sugita and Michael Feig
Pages 228 - 248
Chapter 15
Primary Drug Screening by In-cell NMR Spectroscopy
Stephen Dansereau, David S. Burz and Alexander Shekhtman
Pages 249 - 271
Subject Index
Pages 298 - 305
Publication details
Print publication date
13 Dec 2019
Copyright year
2020
Print ISBN
978-1-78801-217-1
PDF eISBN
978-1-78801-307-9
ePub eISBN
978-1-83916-093-6
About this book
In-cell NMR spectroscopy is a relatively new field. Despite its short history, recent in-cell NMR-related publications in major journals indicate that this method is receiving significant general attention. This book provides the first informative work specifically focused on in-cell NMR. It details the historical background of in-cell NMR, host cells for in-cell NMR studies, methods for in-cell biological techniques and NMR spectroscopy, applications, and future perspectives.
Researchers in biochemistry, biophysics, molecular biology, cell biology, structural biology as well as NMR analysts interested in biological applications will all find this book valuable reading.