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In-cell NMR Spectroscopy: From Molecular Sciences to Cell Biology Editors: Yutaka Ito, Volker Dötsch, Masahiro Shirakawa

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Preliminary content

Front Matter

Preface

Contents

In-cell NMR Spectroscopy: From 2001 to Now

Protein NMR in Escherichia coli

Yansheng Ye and Conggang Li

In-cell NMR in Eukaryotic Cells – I (Introduction from Outside of Cells)

Kohsuke Inomata, Takanori Kigawa and Masahiro Shirakawa

In-cell NMR in Eukaryotic Cells – II (Direct Protein Expression)

Enrico Luchinat and Lucia Banci

Protein Structure Determination in Living Cells from NOE-derived Distance Restraints

Teppei Ikeya, Peter Güntert and Yutaka Ito

In-cell Structure Determination Using Paramagnetic NMR Spectroscopy

Xun-Cheng Su and Thomas Huber

Bioreactor System for In-cell NMR Observation

Noritaka Nishida and Ichio Shimada

¹⁹F In-cell NMR

Yansheng Ye and Conggang Li

Cellular Solid-state NMR Spectroscopy

Reinier Damman, Siddarth Narasimhan, Markus Weingarth and Marc Baldus

In-cell EPR

Anna Bieber and Malte Drescher

In-cell NMR: Spectral Optimization and Population Adjustment by Rational Protein Engineering

Jens Danielsson, Fan Yang and Mikael Oliveberg

Protein Stability and Weak Intracellular Interactions

Alex J. Guseman and Gary J. Pielak

Protein Folding, Maturation and Redox State

Enrico Luchinat and Lucia Banci

All-atom Molecular Dynamics Simulation of Proteins in Crowded Environments

Yuji Sugita and Michael Feig

Primary Drug Screening by In-cell NMR Spectroscopy

Stephen Dansereau, David S. Burz and Alexander Shekhtman

In-cell NMR Spectroscopy of Nucleic Acids

Simon Dzatko, Radovan Fiala, Robert Hänsel-Hertsch, Silvie Foldynova-Trantirkova and Lukas Trantirek

Subject Index

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This book contains 305 pages.

Publication details

DOI: https://doi.org/10.1039/9781788013079
Print publication date: 13 Dec 2019
Copyright year: 2020
Print ISBN: 978-1-78801-217-1
PDF eISBN: 978-1-78801-307-9
ePub eISBN: 978-1-83916-093-6

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About this book

In-cell NMR spectroscopy is a relatively new field. Despite its short history, recent in-cell NMR-related publications in major journals indicate that this method is receiving significant general attention. This book provides the first informative work specifically focused on in-cell NMR. It details the historical background of in-cell NMR, host cells for in-cell NMR studies, methods for in-cell biological techniques and NMR spectroscopy, applications, and future perspectives.

Researchers in biochemistry, biophysics, molecular biology, cell biology, structural biology as well as NMR analysts interested in biological applications will all find this book valuable reading.

From the book series:
New Developments in NMR