About this book
In-cell NMR spectroscopy is a relatively new field. Despite its short history, recent in-cell NMR-related publications in major journals indicate that this method is receiving significant general attention. This book provides the first informative work specifically focused on in-cell NMR. It details the historical background of in-cell NMR, host cells for in-cell NMR studies, methods for in-cell biological techniques and NMR spectroscopy, applications, and future perspectives.
Researchers in biochemistry, biophysics, molecular biology, cell biology, structural biology as well as NMR analysts interested in biological applications will all find this book valuable reading.
Book content
- Brief History of In-Cell NMR Studies
- In-Cell NMR in Bacterial Cells
- In-Cell NMR in Eukaryotic Cells – I Introduction From Outside of Cells
- In-Cell NMR in Eukaryotic Cells – II Intrinsic Protein Expression
- Protein Structure Determination In Living Cells From NOE-Derived Distance Restraints
- Protein Structure Determination in Living Cells from PCS and PRE
- Bioreactor System
- 19F In-Cell NMR
- Whole Cell Solid-State NMR
- In-Cell EPR
- Protein Folding Stability Under Physiological Environments
- Protein Folding Stability with Intracellular Weak Interactions
- Protein Folding and Maturation
- Folding Intermediates Under Physiological Condition
- Macromolecular Crowding
- Molecular Dynamics Simulations of Intracellular Environment
- Post-translational Modifications
- Acetylation
- Protein-Protein Interactions
- Drug-Protein Interactions
- Application to Nucleic Acids
The print version of this book is planned for release on 13 December 2019. Information about this book is subject to change without notice.
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