Modern Biophysical Methods for Screening and Drug Discovery
Genetic Perturbation Methods, from the ‘Awesome Power’ of Yeast Genetics to the CRISPR Revolution
High Throughput Screening Compatible Methods for Quantifying Protein Interactions in Living Cells
Pharmacological and Genetic Screening of Molecularly Characterized Cell Lines
Multidimensional Profile Based Screening: Understanding Biology through Cellular Response Signatures
3D Cell Culture and Dish Based Organogenesis: Optimizing
In vitro Cellular Physiology
Small-molecule-mediated Targeted Protein Degradation for Drug Discovery
Encoded Compound Libraries to Accelerate Small-molecule Therapeutic Discovery
Small-molecule Bioactivity Databases
“So You Want to Run a High-throughput Screen: Do You Know How Much That Costs?”; Costs of High Throughput Screens and How to Fund Them
About this book
High throughput screening remains a key part of early stage drug and tool compound discovery, and methods and technologies have seen many fundamental improvements and innovations over the past 20 years. This comprehensive book provides a historical survey of the field up to the current state-of-the-art. In addition to the specific methods, this book also considers cultural and organizational questions that represent opportunities for future success.
Following thought-provoking foreword and introduction from Professor Stuart Schreiber and the editors, chapters from leading experts across academia and industry cover initial considerations for screening, methods appropriate for different goals in small molecule discovery, newer technologies that provide alternative approaches to traditional miniaturization procedures, and practical aspects such as cost and resourcing. Within the context of their historical development, authors explain common pitfalls and their solutions.
This book will serve as both a practical reference and a thoughtful guide to the philosophy underlying technological change in such a fast-moving area for postgraduates and researchers in academia and industry, particularly in the areas of chemical biology, pharmacology, structural biology and assay development.