The Comet Assay in Toxicology

doi:10.1039/9781782622895

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Preliminary content

Chapter 1

The Comet Assay: A Versatile Tool for Assessing DNA Damage

Mahima Bajpayee, Ashutosh Kumar and Alok Dhawan

Chapter 2

High-throughput Measurement of DNA Breaks and Oxidised Bases with the Comet Assay

Amaya Azqueta, Isabele Campos Costa-Amaral and Andrew R. Collins

Chapter 3

Microplate-based Comet Assay

Elizabeth D. Wagner and Michael J. Plewa

Chapter 4

The Use of Higher Plants in the Comet Assay

Tomas Gichner, Irena Znidar, Elizabeth D. Wagner and Michael J. Plewa

Chapter 5

Methods for Freezing Blood Samples at −80 °C for DNA Damage Analysis in Human Leukocytes

Narendra P. Singh and Henry C. Lai

Chapter 6

Development and Applications of the Comet-FISH Assay for the Study of DNA Damage and Repair

Valerie J. MCkelvey-Martin and Declan J. MCkenna

Chapter 7

The Alkaline Comet Assay in Prognostic Tests for Male Infertility and Assisted Reproductive Technology Outcomes

Sheena E. M. Lewis and Ishola M. Agbaje

Chapter 15

The Comet Assay in Sperm—Assessing Genotoxins in Male Germ Cells

Adolf Baumgartner, Aftab Ali, Charmaine Normington and Diana Anderson

Chapter 16

Genotoxic Effects in Peripheral Blood and Sperm in Humans in Healthy Individuals and Those with Disease States

Diana Anderson, Adolf Baumgartner and Mojgan Najafzadeh

Chapter 17

Application of the Comet Assay in Nanotoxicology

Maria Dusinska, Elise Rundén-Pran, Naouale El Yamani, Lise M. Fjellsbø and Andrew Collins

Chapter 18

Comet Assay – Protocols and Testing Strategies

Andreas Hartmann and Günter Speit

Chapter 19

Imaging and Image Analysis in the Comet Assay

Mark Browne

Chapter 20

Statistical Analysis of Comet Assay Data

David P. Lovell

Subject Index

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Publication details

http://dx.doi.org/10.1039/9781782622895

Print publication date: 07 Oct 2016

Print ISBN: 978-1-78262-287-1

PDF eISBN: 978-1-78262-289-5

ePub eISBN: 978-1-78262-915-3

Citation:

The Comet Assay in Toxicology

Front Matter

Preface

Contents

The Comet Assay: A Versatile Tool for Assessing DNA Damage

High-throughput Measurement of DNA Breaks and Oxidised Bases with the Comet Assay

Microplate-based Comet Assay

The Use of Higher Plants in the Comet Assay

Methods for Freezing Blood Samples at −80 °C for DNA Damage Analysis in Human Leukocytes

Development and Applications of the Comet-FISH Assay for the Study of DNA Damage and Repair

Detection of DNA Damage in Different Organs of the Mouse

Detection of DNA Damage in Drosophila

The Comet Assay: Clinical Applications

Applications of the Comet Assay in Human Biomonitoring

Comet Assay in Human Biomonitoring

Use of Single-cell Gel Electrophoresis Assays in Dietary Intervention Trials

The Application of the Comet Assay in Aquatic Environments