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Practical Laboratory Skills Training Guides: High Performance Liquid Chromatography Author: Brian Stuart


About this book

For those new to this technique, this guide provides basic tips, key skills, awareness and guidance on good practice of High Performance Liquid Chromatography (HPLC). It will help build understanding of the important issues to consider during analysis and how to develop further skills. HPLC is one of the most widely used techniques in industry to separate and analyse compounds through the mass transfer of analytes between stationary and mobile phases. This guide will prove an invaluable introduction to the technique. Starting with a look at the basic theory, the guide goes on to describe HPLC components, system parameters, suitability checks and testing. Later sections cover calibration, problem solving and data handling. High Performance Liquid Chromatography is one of the Practical Laboratory Skills Training Guides, a series that aims to make achieving best practice easy. These invaluable manuals will enable both experienced and inexperienced staff to get the essential basics of any experiment right simply by following the clear and easy to use instructions provided. The guides are written by experienced scientists and include minimal theory, plenty of practical exercises in order to assess competence, and trouble shooting information.

Book content

  • Introduction
  • Key Parameters
  • Quantitative Measures of Column Efficiency
  • Column Resolution
  • The Pump The Injector
  • The Column
  • The Detector
  • HPLC System Parameters and System Suitability Checks
  • System Suitability Checks
  • Equipment Qualification Tests EQ
  • Calibration
  • External Standard
  • Data Handling
  • Keeping Notebooks
This book is print only

Buy paperback £19.95 *
* Exclusive of taxes
This book contains 42 pages.

Publication details

Print publication date: 27 Jan 2003
Copyright year: 2003
Print ISBN: 978-0-85404-483-2