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Protein Phosphorylation Analysis by Electrospray Mass Spectrometry: A guide to concepts and practice Author: Wolf D Lehmann



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This book contains 394 pages.

Publication details


Print publication date
01 Nov 2010
Copyright year
2011
Print ISBN
978-0-85404-185-5
PDF eISBN
978-1-84973-220-8

About this book

Written by an experienced and well-published individual, this unique reference source takes a forward-looking approach. It describes the concepts and practice of protein phosphorylation analysis by tandem mass spectrometry and related techniques. These include purification, enrichment, database searching, other software tools, synthesis, phosphatase treatment, phospho-specific staining methods, isoelectric focusing and element mass spectrometry. The book then goes on to cover the fragmentation behaviour of phosphopeptides in tandem MS (pos+neg ions) and the implementation of the particular features into an analytical strategy. The book ends with a summary and discussion of useful internet and software tools currently available.

Author information

Wolf D. Lehmann studied at the Universities of Bonn and Z?rich before gaining his PhD in 1975. He is currently based at the German Cancer Research Center in Heidelberg, Germany. His areas of expertise include analytical biochemistry, liquid chromatography, mass spectrometry, protein analysis, covalent protein modifications, and peptide sequencing by tandem mass spectrometry. He has published 120 peer-reviewed articles, made numerous book contributions and is on the Advisory Board of the FEBS Journal. He is also a member of the Society of German Chemists, German Society for Mass Spectrometry and American Society for Mass Spectrometry.