Jump to main content
Jump to site search

All chapters
Previous chapter Next chapter


Calcium Digestibility Using In Vivo, In Vitro and Ex Vivo Techniques

As for many other nutrients, Ca digestibility can be determined with in vitro, ex vivo and in vivo techniques. The first group of methods is mainly based on a HCl–pepsin attack, followed by pancreatin–bile extract hydrolysis. After this enzymatic digestion, the digested sample is centrifuged and the Ca in the surnatant represents the soluble fraction. If in the flask where the Ca-containing sample undergoes pancreatin–bile extract digestion, is put a dialysis tube containing water and NaHCO3, we could measure dialyzed Ca. The uptake of Ca by intestinal cells can be estimated supplementing the growth medium of differentiated human tumor cells (HT-29 or Caco-2) with the soluble or dialyzable fraction coming from the in vitro digestion. In vivo techniques include balance studies (both in humans and in animals), studies with labeled Ca or research with bone mineralization as the main endpoint.

Print publication date: 08 Oct 2015
Copyright year: 2016
Print ISBN: 978-1-84973-887-3
PDF eISBN: 978-1-78262-213-0
ePub eISBN: 978-1-78262-729-6