Sample Collection, Storage and Preparation
The first steps of the workflow in metabolomics include sampling, sample pre‐processing and sample preparation. In this chapter, the sample types and sample preparation techniques utilized in metabolomics are presented. The sample preparation methods include homogenization, cell disruption, different extraction methods, such as liquid–liquid extraction, solid–liquid extraction, sample clean‐up and fractionation, and derivatization. Guidelines for the selection of the appropriate sample preparation method based on both the final analysis method and the type of sample and analytes are given.