Selection of Analytical Methodology for Metabolomics
The main challenge in metabolomics analysis is the separation, identification and quantification of a large number of known or unknown metabolites in complex samples. The correct selection of sample collection and preparation method, type of separation methodology and detection are all crucial steps in the analysis of metabolomics. This chapter provides an overview of and general guidelines for strategies involved in the analysis of metabolomics, and different chromatographic techniques used in metabolomics studies are briefly presented. The methods are compared and the main features of the separation methods are listed. Finally, general conclusions and future perspectives are provided.