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Synthesis and Properties of Oligonucleotides Forming G-quadruplexes

The development of methodology for the chemical synthesis of oligonucleotides is the first step for producing not only the natural G-quadruplex sequences but also a large number of modified G-quadruplexes and other G-based supramolecular motifs; these methodologies will be summarized in this chapter. Natural or chemically modified G-motifs can be characterized by a variety of techniques. This chapter will not give an exhaustive list of methodologies, but simply shows how it is possible to gain structural information on G-quadruplexes by means relatively uncommon techniques such as: (i) differential pulse voltammetry; (ii) atomic force microscopy; (iii) dynamic light scattering; (iv) solution X-ray scattering; (v) temperature gradient gel-electrophoresis; (vi) Langmuir-Blodgett technique. Many other techniques are more usually adopted in G4 characterization (NMR, Scanning Tunnel Microscopy, Circular Dichroism, X-ray diffraction …) and examples of such applications can be found in the contributions to the other chapters of this book.

Publication details

Print publication date
13 Nov 2012
Copyright year
2013
Print ISBN
978-1-84973-460-8
PDF eISBN
978-1-84973-695-4