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The Preparation, Optimization and Characterization of Viral Protein Complexed Liposomes.

The present study is aimed at exploiting the potential of liposomal nanomedicines in the design of an intranasal HBsAg vaccine and describes the preparation, optimization and characterization of the same. In order to enhance the nasal uptake of the liposomal carrier the mucosal M cell binding protein haemagglutinin (HA) from the influenza virus was complexed onto the liposomal surface. The liposomal formulations were prepared by the dehydration-rehydration-vesiculation method. They were optimized and characterized for various in vitro parameters and the optimized liposomal formulations were again selected for the conjugation of HA on to its surface by the carbodimide coupling method. Initially, the formulations were prepared and various process and parameters that could affect the preparation and properties of the system(s) were identified, characterized and studied.

Print publication date: 04 Apr 2012
Copyright year: 2012
Print ISBN: 978-1-84973-378-6
PDF eISBN: 978-1-84973-524-7