CHAPTER 5: Experimental Conditions and Processing

In this chapter, basic experimental conditions and data processing that are necessary to obtain good quality one- and two-dimensional spectra will be presented. In particular, the influence of fundamental acquisition parameters on the way in which nuclear magnetic resonance (NMR) data in the time domain are collected will be discussed together with suitable suggestions in order to avoid common pitfalls that may decrease the information content in the NMR spectra. Computational manipulations of the NMR data in the time and frequency domain for resolution and sensitivity enhancement will be discussed. In addition, several practical recommendations and useful pulse sequences to obtain proper one- and two-dimensional NMR spectra for quantitative analysis will be given.