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Chapter 7.2

Fluorescence Lifetime Imaging applied to Microviscosity Mapping and Fluorescence Modification Studies in Cells

Fluorescence lifetime imaging (FLIM) is a key optical technique for imaging the photophysical environment of fluorescent probes in vivo. In addition, it also provides information in fluorescence enhancement studies that intensity imaging alone could not provide. We review the principles and recent advances in the application of the techniques, instrumentation and molecular probe development.

Print publication date: 30 Nov 2011
Copyright year: 2012
Print ISBN: 978-1-84973-014-3
PDF eISBN: 978-1-84973-291-8
From the book series:
Drug Discovery