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Chapter 12

Culture Media for Detection and Enumeration of “Total” Enterobacteriaceae, Coliforms and Escherichia coli from Foods

Recent developments in enrichment and selective media for the isolation and enumeration of “total” Enterobacteriaceae, coliforms, faecal coliforms and E. coli from foods are described and effects of time and temperature of incubation discussed. Coliforms and E. coli are both important indicators of food contamination; both therefore need to be detected in the same medium. Several attempts have been made to detect coliforms and E. coli simultaneously and novel methods have been introduced, based on the detection of β-d-galactosidase (β-D) and β-D-glucuronidase (GUD) using enzymatic methods. Factors to be considered in the selection of media for this group of organisms are noted, based on the results of comparative studies.

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06 Dec 2011
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