Chemical Derivatization for Polar Metabolome Analysis
A typical metabolome sample contains many polar metabolites. Chemical derivatization of these metabolites offers a viable option to improve their analysis. Because of diverse chemical structures of metabolites, development of new or improved derivatization reagents and methods for analyzing polar metabolites is an active research area. Various derivatization methods targeting different functional groups have been reported. This chapter highlights some of the methods for targeted polar metabolite analysis. For untargeted analysis, a high-performance chemical isotope labeling approach for analyzing both polar and less-polar metabolites together in a reversed-phase LC–MS setup is described.