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CHAPTER 6

Discovery and Use of Natural Products and Derivatives as Chemical Probes

Among various structurally and functionally diverse natural products, some compounds modulate protein–protein interactions (PPIs). The anti-tumour macrolide aplyronine A is a PPI stabilizer between tubulin and actin and prevents spindle formation and mitosis. As a case study of using natural products as chemical probes, target identification and mode of action studies of aplyronines are presented. A new methodology using an amidopyrene tag and label-assisted laser desorption/ionization mass spectrometry (LA-LDI-MS) for binding position analysis of ligands on target biomacromolecules is also highlighted.

Publication details

Print publication date
26 Nov 2020
Copyright year
2021
Print ISBN
978-1-78801-589-9
PDF eISBN
978-1-83916-074-5
ePub eISBN
978-1-83916-084-4

From the book series:
Chemical Biology