Primary Drug Screening by In-cell NMR Spectroscopy
The successful application of in-cell NMR spectroscopy for primary drug high-throughput screening (HTS), by direct and indirect target engagement in prokaryotes is reviewed. We discuss approaches for identifying drug-binding targets, mapping the target interaction surface and methodologies to rapidly screen small-molecule libraries, as well as nascent strategies for primary drug HTS in eukaryotic cells, including novel methods of target and drug delivery to cells.