Jump to main content
Jump to site search

All chapters
Previous chapter Next chapter

Chapter 13

Protein Folding, Maturation and Redox State

The direct protein expression approach described in Chapter 4 is ideally suited for observing cellular functional processes involving protein folding and maturation, cofactor binding and redox state regulation by in-cell NMR in human cells. In this chapter, some applications of in-cell NMR to investigate protein functional processes in HEK293T cells are described, including the maturation of superoxide dismutase 1, from zinc binding to copper delivery and disulphide bond formation, the effect of disease-linked mutations and the role of the specific copper chaperone, the redox-dependent folding of two small mitochondrial proteins as a function of specific redox partners and the effect of exogenous molecules on the protein redox state.

Publication details

https://doi.org/10.1039/9781788013079-00207
Print publication date
13 Dec 2019
Copyright year
2020
Print ISBN
978-1-78801-217-1
PDF eISBN
978-1-78801-307-9
ePub eISBN
978-1-83916-093-6
From the book series:
New Developments in NMR