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CHAPTER 4

Capillary Electrophoresis–Mass Spectrometry for Metabolomics Using New Interfacing Designs

In metabolomics, capillary electrophoresis–mass spectrometry (CE-MS) using electrospray ionization (ESI) has become a useful analytical technique for the profiling of highly polar and charged metabolites. In general, a co-axial sheath-liquid interface is employed for coupling CE to MS. In this approach, the ESI process is mainly dictated by the sheath-liquid and not the CE effluent. A wide range of sheath-liquid compositions with or without additives can be explored in order to improve the ESI process, and as such, this interfacing approach provides a high degree of flexibility. Though CE-MS using a sheath-liquid interface can be used in a rather robust way for metabolic profiling studies, the significant dilution of the CE effluent (10–100 nL min−1) by the sheath-liquid, which is generally provided at a flow-rate between 2 and 10 µL min−1, can be considered as a major drawback for global metabolic profiling. Recent advances in interfacing designs allow CE-MS to be used under low flow-rate conditions. In this context, the sheathless porous tip interface, the flow-through microvial interface and the electrokinetic-driven sheath-liquid interface have emerged as important recent developments. In this chapter, the utility of CE-MS using these new interfacing designs is demonstrated by representative examples in the fields of biomedical and clinical metabolomics.

Publication details


Print publication date
13 Jul 2018
Copyright year
2018
Print ISBN
978-1-78801-104-4
PDF eISBN
978-1-78801-273-7
ePub eISBN
978-1-78801-484-7