Transmission Cryo-electron Microscopy in Drug Discovery
Transmission cryo-electron microscopy has recently experienced a technical and methodological revolution that has made it the most promising structural biology technique of the next decade. It is now possible to solve structures of biological macromolecular complexes at near-atomic resolution. It is therefore now feasible to include cryo-electron microscopy among the techniques that can be used for structure-based drug design. In this chapter, we will summarise the latest improvements that made the revolution in cryo-electron microscopy possible, show and discuss the potential of cryo-electron microscopy in drug design, and finally discuss the present limits and future possible improvements in cryo-electron microscopy.