Mass Spectrometry in Biophysics: from High Throughput Screening to Structural Biology
Mass spectrometry (MS) is playing an increasingly crucial role within the biophysical community in the study of protein architecture, dynamics and interactions. The impressive technological and methodological advances in recent years have widened the applicability of mass spectrometry from straightforward high throughput screening (HTS) to studying increasingly complex problems focused around biopolymers of interest. This chapter covers current state of the art of MS techniques in biophysical research for academic and industrial researchers working in the biophysical arena. Within MS-based applications in biophysics, the study of protein structure and dynamics by MS complements other biophysical tools and opens the possibility to study proteins under near-physiological conditions and resolve higher order structure assemblies. The study of ligand–protein and protein–protein interactions (PPIs) has a central role in current screening approaches and it will be covered in some detail.