Receptor-based NMR Techniques in Drug Discovery
Nuclear Magnetic Resonance spectroscopy is a highly resolutive method for drug-discovery as it provides information about drug association to a target protein, over a wide range of affinities and with atomic resolution. The binding event affects many NMR observables that are sensitive to the chemical environment and to putative changes in the structure/dynamics upon binding. The versatility and robustness of this technique explains why pharmaceutical and research groups routinely incorporate NMR spectroscopy into their high-throughput ligand screening strategies. In this chapter, we pay attention to the receptor-based NMR experiments that monitor the target protein to quantitatively describe and validate the small-compound binding phenomena.