Jump to main content
Jump to site search
SCHEDULED MAINTENANCE Close the message box

Maintenance work is planned for Monday 16 August 2021 from 07:00 to 23:59 (BST).

Website performance may be temporarily affected and you may not be able to access some PDFs or images. If this does happen, refreshing your web browser should resolve the issue. We apologise for any inconvenience this might cause and thank you for your patience.


All chapters

Chapter 3

The Mesh&Collect Pipeline for the Collection of Multi-crystal Data Sets in Macromolecular Crystallography

The advent at 3rd generation synchrotron sources of extremely brilliant beamlines for macromolecular crystallography (MX) has meant that for systems which produce only small crystals, the practice of multi-crystal data collection, in which partial data sets collected from a series of crystals of the same target are merged to produce a final, complete data set, is enjoying a renaissance. In principle such experiments are straightforward. All that is needed is to mount all the crystals required on the sample support, to collect the partial data sets in series and then to merge them to produce the final data set. However, multi-crystal data collection is actually fraught with difficultly. The positions of the crystals on the sample support must be determined; individual partial data sets should be collected without inducing too much radiation damage; and the correct choice of partial data sets to merge in order to produce the best final data set must be made. Here, we describe an automatic pipeline, Mesh&Collect, which facilitates multi-crystal experiments, present newer developments designed to improve the quality of the final data sets obtained, give examples of the successes that can be obtained and describe the pitfalls to be avoided in such experiments.

Publication details


Print publication date
21 Jun 2018
Copyright year
2018
Print ISBN
978-1-78262-728-9
PDF eISBN
978-1-78801-050-4
ePub eISBN
978-1-78801-477-9
From the book series:
Chemical Biology