Chemiluminescence and Fluorescence Optical Biosensor for the Detection of Aflatoxins in Food
The effective analysis of food contaminants, especially toxins, requires the development of innovative bioanalytical devices or biosensors that are precise, sensitive, specific, rapid, and easy to use to, in order to meet the increasing demand for legislative actions on food safety and control. Optical biosensors, are a powerful alternative to conventional analytical techniques. They enable the highly sensitive, real-time, and high-throughput analysis of food toxins without needing extensive sample preparation. Among the optical biosensing techniques, chemiluminescence and fluorescence are used to detect food toxins as they are highly sensitive and amplification of the optical signal is possible without much interference. The use if these contactless sensors eliminates backward influence of the measuring device on the object of measurement. Aflatoxins are food toxins which affect almost all food commodities. They are potentially hazardous carcinogens and therefore their early and sensitive detection is greatly needed. In this chapter, we focus on the development of optical biosensing techniques for ultrasensitive analysis of aflatoxin M1 in milk and milk products based on chemiluminescence and fluorescence detection.