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Towards Simultaneous Force and Resistive Pulse Sensing in Protein Nanopores Using Optical Tweezers

Protein nanopores are highly suitable for single-molecule detection. They offer more reproducible, cost effective and well defined structures than solid-state alternatives with architectures often known from x-ray crystallography studies with sub-nanometer precision. Here we present a self-assembling hybrid nanopore system consisting of a protein nanopore embedded in a lipid membrane, supported across the tip of a nanopipette. Here, we show the insertion of Staphylococcus aureus toxin α-hemolysin into the supported membrane and the voltage-driven transport of single-stranded DNA homopolymers. Orientation of the nanopipette perpendicular to the optical trapping axis will allow for high resolution force measurements of macromolecular transport through protein nanopores.

Publication details


Print publication date
07 Sep 2012
Copyright year
2013
Print ISBN
978-1-84973-416-5
PDF eISBN
978-1-84973-527-8
From the book series:
Special Publications