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Nitroxide spin labels: fabulous spy spins for biostructural EPR applications

Characterizing proteins in action requires appropriate biophysical techniques sensitive to protein motions. One of the technique dedicated to monitor protein dynamics is Site-Directed Spin Labelling combined with EPR spectroscopy (SDSL-EPR). The main purpose of this chapter is to describe and illustrate the different strategies based on the use of nitroxide spin labels either as reporters or as a means to measure inter-label distances. The complementarity of these different approaches to answer biological questions will be addressed. The objective is also to give non-specialist readers an overview of the recent developments in the field of SDSL-EPR dedicated to the study of protein dynamics. A particular emphasis will be devoted to describe the design and application of new nitroxide spin labels that allow overcoming the limitations of the classical ones.

Publication details

Print publication date
06 Nov 2018
Copyright year
2019
Print ISBN
978-1-78801-372-7
PDF eISBN
978-1-78801-388-8
ePub eISBN
978-1-78801-688-9