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CHAPTER 8

Stable Isotope Labeling of Glycoproteins for NMR Study

In the study of glycoproteins by NMR spectroscopy, stable isotope labeling is a challenging but important step. Labeling by metabolic means is useful, but choice of production vehicles (mammalian or non-mammalian expression systems) and of medium (or diet) are critical for obtaining functional glycoproteins with high isotope enrichment and with the appropriate glycoform framework. Metabolic labeling coupled with in vitro enzymatic reactions enables remodeling or modification of the NMR target glycoform. This chapter outlines recent progress in isotope labeling techniques of glycoproteins using mammalian and non-mammalian expression systems.

Print publication date: 17 May 2017
Copyright year: 2017
Print ISBN: 978-1-78262-310-6
PDF eISBN: 978-1-78262-394-6
ePub eISBN: 978-1-78801-128-0
From the book series:
New Developments in NMR