Hyaluronic Acid-Based Nanofibrous Scaffolds Incorporating Nigella sativa Methanolic Extract with Multifunctional Antimicrobial, Antioxidant, An-ti-inflammatory, and Anticancer Properties for Advanced Wound Healing

Abstract

The present study reports the fabrication and characterization of hyaluronic acid nanofibers in-corporated with Nigella sativa methanolic extract (HA/NSME-nanofibers) for potential skin therapeutic applications. The HA/NSME-nanofibers were successfully electrospun and systemati-cally characterized in terms of morphology, mechanical properties, surface wettability, and chemical interactions. The bioactive properties of the fibers, including antibacterial, antifungal, antioxi-dant, anti-inflammatory, and anticancer activities, were assessed, demonstrating promising multi-functional behavior suitable for wound healing and skin tissue engineering. Incorporation of plant-derived bioactive compounds into biopolymeric fibers provides a synergistic platform for developing advanced wound dressings.