Five new phenylpropanoid amides from the seeds of Cannabis sativa L. and their Anti-flammatory activity

Abstract

Out of the 70% ethanol extract of Cannabis sativa L. seeds, five new phenylpropanoid amides, named huomarenamides C-H (1–5), in addition to nine previously documented compounds (6-14), were obtained. The structural accuracy of the new compounds was ensured by unambiguously determining their absolute configurations using spectroscopic analyses and the ECD method. Meanwhile, the characterization of known compounds relied on a meticulous comparison of the known compounds' 1D NMR data with publications reports. The neuroinflammatory suppressive potential of each compound was gauged within a non-cytotoxic milieu through the LPS-activated BV2 microglial inflammation paradigm. The outcomes demonstrated that compound 4 possessed remarkable anti-neuroinflammatory activity (IC50 = 6.81 ± 3.04 μM).