An in vitro organ-on-chip model for studying neuron–keratinocyte interactions in sensory response through electrophysiology

Abstract

This study introduces a human-relevant in vitro model using iPSC-derived sensory neurons and keratinocytes in MEA-integrated microfluidic chips. Neurons expressed nociceptor markers, showed TRPV activity, and formed contacts with keratinocytes. Stimuli evoked electrophysiological responses, highlighting neuron–keratinocyte interactions relevant to pruritus, pain, and skin disorders, supporting therapeutic development.