Plum derived nanovesicles alleviate DSS-induced colitis by modulating gut-liver axis function

Abstract

Inflammatory bowel disease (IBD) is a digestive tract disorder characterized by chronic intestinal inflammation, leading to various symptoms and complications. In this study, we investigated the protective effects of plum derived nanovesicles (PNVs) on the gut-liver axis in a mouse model of dextran sulfate sodium (DSS)-induced acute colitis. When PNVs were administered orally once daily for a week to mice with DSS-induced colitis, they restored colon length, repaired colonic epithelial damage, and regulated intestinal microbial composition.Nanoparticle tracking analysis (NTA) and transmission electron microscopy (TEM) confirmed that PNVs were 100-200 nm in size and successfully separated. PNVs reached multiple organs safely and restored impaired intestinal function both in vivo and in vitro. Moreover, PNVs inhibited oxidative stress marker proteins and significantly reduced DSS-induced intestinal permeability, endotoxemia, and bowel and liver inflammation. These findings suggest that PNVs may alleviate IBD and protect the gut-liver axis through their antioxidant and antiinflammatory properties.