We report an adapted Transwell® assay for robust and quantitative analysis of chemotactic migration in Dictyostelium discoideum. This interface-based platform identifies soluble folate as a chemical attractant and reveals defective migration in Far1-deficient cells, enabling rapid discrimination of stimulus-specific and genetic determinants of amoeboid motility.
M. Bravo, A. Ramos, D. Rojas, I. Pérez, A. E. Marcoleta and F. P. Chávez, Anal. Methods, 2026, Accepted Manuscript , DOI: 10.1039/D6AY00051G
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